Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Cell Signaling Technology Phosphate Buffered Saline (PBS-20X) 1000 ml
Phosphate Buffered Saline (PBS-20X) 1000 ml
Cell Signaling Technology Phosphate Buffered Saline (PBS-1X) pH7.2 (Sterile) 10 ml
Phosphate Buffered Saline (PBS-1X) pH7.2 (Sterile) 10 ml
Chem-Impex International, Inc. MOPS sodium salt | 71119-22-7 | MFCD00064350 | 25G
MOPS sodium salt, 71119-22-7, MFCD00064350, 25G
Biotang Inc HEPES Protein Extraction Buffer (5X), 500 ml
HEPES Protein Extraction Buffer (5X), 500 ml.
Biotang Inc Tricine-buffered Saline (5X) pH 7.0, 250 ml
Tricine-buffered Saline (5X) pH 7.0, 250 ml.
Biotang Inc E. Coli Lysis Buffer (Tris-HCl 50 mM,1% Triton,EDTA 1 mM, Beta-mercaptoethanol 10 mM), 250 ml
E. Coli Lysis Buffer (Tris-HCl 50 mM,1% Triton,EDTA 1 mM, Beta-mercaptoethanol 10 mM), 250 ml.
Quality Biological Inc TBE Buffer 10X, 4 Liters
TBE (TRIS Borate EDTA) is often used in procedures involving nucleic acids, the most common being electrophoresis. ¿ Tris-acid solutions are effective buffers for slightly basic conditions, which keep DNA deprotonated and soluble in water. ¿ Applications: TBE Buffer, 1X is used for DNA electrophoresis. ¿ TBE Buffer, 0.5X is used for electrophoresis onto DEAE cellulose membrane. ¿ TBE Buffer, 2X is used for polyacrylamide and agarose electrophoresis. ¿ TBE is also used for other nucleic acid electrophoresis procedures such as Northern and Southern blots. ¿ Storage Conditions: 15 to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ DNase, RNase, and Protease Tested ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ ¿ Max Shelf Life: 36 months
FUJIFILM BIOSCIENCES INC PBS 1X-Dulbecco's Phosphate Buffered Saline Solution - Liquid
PBS 1X Dulbecco's Phosphate Buffered Saline Solution can be used to provide a buffer system to maintain cell culture media in the physiological pH range, or to irrigate, transport, or dilute fluid while maintaining cell tonicity and viability.
Zymo Research Corporation DNA Elution Buffer, 4 ml
This can be used with any of the DNA purification for DNA elution from columns, plates, and magbeads.