Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Boston Bioproducts Inc HEPES Buffer (0.5 M, pH 7.0) - 250ml
HEPES Buffer (0 5 M pH 7 0) - 250ml (For Research Use and Further Manufacturing Only)
![Bioworld ACES (N-[carbamoylmethyl]-2-Aminoethane-sulfonic acid) Buffer, 0.5M, pH 6.2, 100 mL](https://assets.fishersci.com/TFS-Assets/CCG/product-images/50255310.jpg-250.jpg)
Bioworld ACES (N-[carbamoylmethyl]-2-Aminoethane-sulfonic acid) Buffer, 0.5M, pH 6.2, 100 mL
Synonym:N-(2-Acetamido)-2-aminoethanesulfonic acid; N-(Carbamoylmethyl)-2-aminoethanesulfonic acid; N-(Carbamoylmethyl)taurineACES is one ofGood's buffersdeveloped in the 1960s to provide buffers withpHranging from 6.15-8.35 for use in various applications. Azwitterionicbuffer with apKaof 6.9, it is often used as abuffering agentinbiologicalandbiochemicalresearch. ACES had been used to develop buffers for bothagaroseandpolyacrylamide gel electrophoresis.A saturated solution contains 0.22 M at 0°C. ACES binds Cu2+, but not significantly Mg2+, Ca2+or Mn2+.Appearance: LiquidHS Code : 29241900Melting Point: >220 °C (dec.)(lit.)PH Value: 6.0
Hampton Research HR2-902-01/StockOptions HEPES #01 pH 6.8, 185 ml
HR2-902-01/StockOptions HEPES #01 pH 6.8, 185 ml. Please note items from this supplier are non-returnable.
Medchemexpress LLC PIPES (1,4-Piperazinediethanesulfonic acid) | 5625-37-6 | ≥99.0% | 5 G
PIPES (1,4-Piperazinediethanesulfonic acid) is an important component of PIPES buffer agent used in biochemistry. It is for research use only.
- Important component of buffer agent
- Prevents glutaraldehyde fixation-induced lipid loss and artifacts when used in buffer
Bioworld MOPS-SDS Running Buffer (20X), 1 L
MOPS-SDS Running Buffer for Protein Gel electrophoresisSDS running buffer with MOPS and Tris (pH 7.7)20X concentrationAll bioWORLD running buffers are prepared with high quality reagentsSpecifications:Ready to use, just dilute in distilled water. Save time and ensure reproducible results.1X contains: 50mM MOPS, 50mM Tris base, 1mM EDTA, and 0.1% SDS
Boston Bioproducts Inc HEPES Buffer (0.5 M, pH 7.0, pH Adjusted with KOH) - 250ml
HEPES Buffer (0 5 M pH 7 0 pH Adjusted with KOH) - 250ml (For Research Use and Further Manufacturing Only)
Medchemexpress LLC MES potassium | 39946-25-3 | 99.88% | 100 G
MES (2-Morpholinoethanesulphonic acid) potassium is an amphoteric ion buffer with a buffer capacity ranging from pH 5.5 to 7.0. It is a Good's buffer and is widely used in biochemistry and molecular biology experiments such as cell culture, enzyme activity determination, electrophoresis, and protein studies.
- Amphoteric ion buffer
- Buffer capacity ranging pH 5.5-7.0
- Widely used in biochemistry and molecular biology experiments (e.g., cell culture, enzyme activity determination, electrophoresis, protein studies)
- Zwitterionic buffer, provides effective buffering capacity around pH 6.15
- Pka value changes with temperature, requiring consideration for different temperatures
- Exhibits good buffering properties in biological experiments
- Shows poor affinity with metal ions (e.g., Mg2+, Ca2+, Mn2+), suitable for studies involving metal ions
![Alkali Scientific MOPS, Sodium Salt [3 (N Morpholino) propanesulfonic acid sodium salt], 2.5 Kg](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659991.jpg-250.jpg)
Alkali Scientific MOPS, Sodium Salt [3 (N Morpholino) propanesulfonic acid sodium salt], 2.5 Kg
MOPS (3-(N-Morpholino)propanesulfonic acid sodium salt) is a highly effective biochemical reagent used for pH buffering in a variety of biological, biochemical, and chemical processes. This 2.5 kg container is ideal for high-throughput laboratories that require a stable, consistent pH environment. MOPS is commonly used in experiments related to protein and nucleic acid analysis, enzyme activity assays, and electrophoresis. Its ability to maintain a steady pH of 7.0–7.5 makes it indispensable in cell culture and biological research where pH fluctuations could interfere with sensitive processes. It is also a standard reagent for maintaining pH in buffers for molecular biology applications.
Sigma Aldrich Fine Chemicals Biosciences MOPS BioUltra, for molecular biology, >=99.5% (T) | 1132-61-2 | MFCD00006183 | 100G
MOPS BioUltra, for molecular biology, >=99.5% (T) | Purity: >=99.5% (T) | Mol Wt: 209.26 | 1132-61-2 | MFCD00006183 | 100G
Gold Biotechnology Inc MES Free Acid 1 kg
MES is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al It is a zwitterionic morpholinic buffer that is useful for a pH range of 5 5 6 7 and commonly used for cell culture media as a running buffer in electrophoresis and for protein purification in chromatography MES lacks the ability to form a complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions MES is often used in buffered culture media for bacteria yeast and mammalian cells It is toxic towards plants at high concentrations but can be used in culture media at lower concentrations for plants Due to low ionic mobility MES is regarded as an excellent buffer for use in capillary electrochromatography MES is often employed as a substitute for the highly toxic cacodylate as well as a substitute for the non-zwitterionic buffers citrate and malate
![Alkali Scientific PIPES Disodium Salt [Piperazine N,N' bis(2 ethanesulfonic acid)disodium salt], 100 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659862.jpg-250.jpg)
Alkali Scientific PIPES Disodium Salt [Piperazine N,N' bis(2 ethanesulfonic acid)disodium salt], 100 G
PIPES Disodium Salt (Piperazine N,N' bis(2 ethanesulfonic acid) disodium salt) is a biochemical reagent widely used in laboratory buffer solutions. This 100g formulation is perfect for maintaining a stable pH in various biological and biochemical assays. The disodium salt form provides a slightly stronger buffering capacity compared to its other forms, making it ideal for sensitive experiments such as protein electrophoresis and enzyme activity assays. It helps maintain a consistent pH of approximately 7.0, which is essential for stabilizing biological molecules during lab procedures. PIPES disodium salt is a reliable buffer in cell culture, microbiology, and biochemical studies.
Bioworld ADA (N-(2-acetamido)iminodiacetic acid) Buffer, 0.2M, pH 6.5, 250 mL
Synonym:ADA-Na,N-(2-Acetamido)iminodiacetic acid monosodium salt,N-(Carbamoylmethyl)iminodiacetic acid monosodium saltpH: useful range: 6.0 - 7.2Warning: For laboratory use only.Not fit for agricultural, household, clinical use or human consumption. PH Value: : 6.0 - 7.2
![Research Products International Corp HEPES, Sodium Salt [N-(2-Hydroxyethyl) piperazine-N'-(2-ethanesulfonic acid)], 500 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-H75050-500.04.JPG-250.jpg)
Research Products International Corp HEPES, Sodium Salt [N-(2-Hydroxyethyl) piperazine-N'-(2-ethanesulfonic acid)], 500 Grams
pKa (20°C): 7.45-7.65
pH Range: 6.8-8.2